The Location of Chitinasome on The Surface of Chitinibacter tainanensis

Abstract

A novel chitin-degrading aerobe, Chitinibacter tainanensis, was proved to produce N-acetylglucosamine. Chitinasome on the surface of the aerobe was identified to degrade chitin. It was suggested that chitinasome was a complicated protein with several subunits, as well as endochitinase and NAGase activities. While the related locations of chitinasome, cell wall and cell membrane were not clear. In this study, cell wall, as well as outer cell membrane, was attacked to identify the location of chitinasome. The result suggested that chitinasome was associated with outer membrane and penetrated out of cell wall to degrade chitin. The structure model was also performed.

• Chitinibacter tainanensis
• Chitinasome
• Cell wall

摘要

Chitinibacter tainanensis可直接分解幾丁質成為N-乙醯葡萄糖胺，先前的研究已證實係利用菌體表面的幾丁質?體(Chitinasome)來分解幾丁質，電子顯微鏡觀察Chitinibacter tainanensis，可看見菌體表面有突起的結構，推測可能就是Chitinasome。Chitinasome為具有Endochitinase與N-acetylglucosaminidase(NAGase)的多次單元複合體蛋白質，與菌體表面相對位置的細胞壁與細胞膜的交互作用尚未清楚。本研究利用Lysozyme處理位於對數生長期的Chitinibacter tainanensis菌體，發現菌體NAGase活性未改變，再經Lauroylsarcosine萃取則溶出的NAGase大幅提升，因此推測Chitinasome係附著於細胞外膜上，穿透細胞壁與幾丁質結合進行分解作用。

• Chitinibacter tainanensis
• 幾丁質?體
• 細胞壁

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