Description Detail

Vol.25, No.1(2018-11)(1-6) 
Cytocompatibility and Antibacterial Activity of Double Cationic Chitoson Quaternary Phosphonium Derivatives
雙陽離子性四級鏻化幾丁聚醣衍生物細胞相容性與抗菌活性之研究
Dai-Ling Tsai, Cheng-Wei Lin, Li-Wei Zheng, Zi-Xuan Zheng, Chi-Hsiung Jou
蔡岱凌, 林政緯,鄭力瑋, 曾子軒, 周啟雄
In this study, the O-(2-hydroxy) propyl-3-methyl diallyl ammonium chitosan salt (O-HDACS) were applied to make reaction of chitosan and N-epoxy-2,3-propyl-N-methyl-N,N-diallylammonium methyl sulfate (MDAA). Afterwards, four double cationic quaternary phosphoniun chitosan derivatives (DCQPCS) were synthesized by O-HDACS and four (N-carboxyalkyl) triphenylphosphonium of compounds (CATPPs). These DCQPCS were characterized by FT-IR. Antioxidant activity were evaluated by DPPH (2,2-diphenyl-1-picryhydrazyl) radical scavenging assay. The cytocompatibility was tested by L929 fibroblast to evaluate proliferation and cytotoxicity. In addition, the antibacterial activity was tested against Staphylococus aureus by calculation of the minimum bactericidal concentrations (MBC).
本研究係以幾丁聚醣與N-環氧基2,3丙基-N-甲基-N,N-二烯丙基甲基磺酸化銨 (MDAA)反應得到O-(2-羥基) 丙基-3-甲基-二丙烯基氨幾丁聚醣磺酸化物(O-HDACS),之後O-HDACS分別與4種N-(羧烷基)三苯基溴化鏻化合物(CATPP)進行醯氨化反應而得4種雙陽離子性四級鏻化幾丁聚醣衍生物(DCQPCS)。DCQPCS的特性以傅立葉轉換紅外光光譜儀(FT-IR)分析其分子結構,細胞相容性以L929纖維母細胞測試來評估其細胞成長活性與細胞毒性。抗氧化性則以DPPH游離基螯合能力來評估,另外,以金黃色葡萄球菌(Staphylococus aureus)測試最小殺菌濃度來評估抗菌活性。
Quaternary Phosphoniun Chitosan Derivatives, L929 Fibroblast, Cytocompatibility, MTT Test Antioxidant Activity, Staphylococus Aureus, Antibacterial Activity
四級鏻化幾丁聚醣衍生物, L929老鼠纖維母細胞, 細胞相容性, MTT測試, 抗氧化性, 金黃色葡萄球菌, 抗菌活性
Year Volume
2020 27.1 | 27.2
27.3 |
2019 26.1 |
2018 25.1 |
2017 24.1 | 24.2
24.3 | 24.4
24.5 | 24.6
24.7 | 24.8
24.9 |
2016 23.1 | 23.2
23.3 | 23.4
23.5 | 23.6
23.7 | 23.8
2015 22.1 | 22.2
22.3 | 22.4
22.5 |
2014 21.1 | 21.2
21.3 |
2013 20.1 | 20.2
20.3 | 20.4
2012 19.1 | 19.2
19.3 | 19.4
2011 18.1 | 18.2
2010 17.4