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Vol.20, No.4(2013-)(193) 
Antibacterial Activity and Antioxidant Activity of Fluorescein Grafted with N-diallylammonium Chitosan Derivate
螢光黃於N-二丙基幾丁聚醣衍生物之抗菌性及抗氧化性
C. H. Jou, F. C. Liang, Y. T. Tsai, J. H. Wu, N. X. Su, R. C. Fu, C. C. Wang, W.T. Huang, C.Y. Cheng
周啟雄, 梁芳誠, 蔡雁霆, 吳宗翰, 蘇楠雄, 傅菖濡, 王啟忠 黃仴婷 鄭棋尹
In this research, the N-(2-hydroxyl) propyl-3-methyl diallyl quaternary ammonium chitosan salts (HMDC) of modified chitosan derivates, which were synthesized by chitosan with glycidy methyl diallyl ammonium salt (GMDAS). Furthermore, HMDC was modified by the reaction with fluorescein of fluorescent dyes to produce HMDC-FL of photosensitive fluorescent chitosan derivatives. HMDC-FL of photosensitive fluorescent chitosan derivatives was examined by using Fourier transform infrared spectroscopy (FTIR) to determine the chemical structures. The grafted ratio of fluorescein grafted with HMDC was determined and calculated by UV/Vis Spectrophotometer,and antioxidant activity were evaluated by DPPH (2,2-diphenyl-1-picryhydrazyl ) radical scavenging assay. The antibacterial activities of HMDC and HMDC-FL tested against two bacteria: Staphylococus aureus and Klobsiella pneumonia. Antibacterial activity was evaluated by minimum bactericidal concentration (MBC). The results showed the antibacterial activity of HMDC-FL was higher than HMDC.
本研究係以自行合成的N-(2-羥基)丙基-3-甲基二丙烯基四級銨鹽 glycidyl-methyl-diallyl amonium salt (GMDAS)修飾幾丁聚醣,進行N-(2-羥基) 丙基-3-甲基丙二烯基四級銨鹽幾丁聚醣衍生物(HMDC)合成,再將螢光黃(fluorescein)與HMDC進行修飾反應,而得HMDC-FL具光感性螢光幾丁聚醣衍生物。以傅立葉紅外線光譜分析儀(FT-IR)分析螢光黃、HMDC及HMDC-FL光感性螢光幾丁聚醣衍生物,紫外光/可見光吸收光譜儀(UV/Vis Spectrophotometer)計算出螢光染料的接枝率及測定DPPH自由基清除能力。HMDC及HMDC-FL分別以金黃色葡萄球菌 (Staphylococcus aureus) 和肺炎桿菌 (Klebsiella pneumonia) 測試最小殺菌濃度(MBC)來評估抗菌活性。研究結果發現HMDC-FL對金黃色葡萄球菌及肺炎桿菌的抗菌活性高於HMDC。
chitosan, fluorescein, antibacterial activity, antioxidant activity
幾丁聚醣、螢光黃、抗菌性、抗氧化
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